PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT5G54070.1
Common NameAT-HSFA9, HSF21, HSFA9, MJP23.4
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family HSF
Protein Properties Length: 331aa    MW: 38146.2 Da    PI: 5.373
Description heat shock transcription factor A9
Gene Model
Gene Model ID Type Source Coding Sequence
AT5G54070.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HSF_DNA-bind108.45.3e-34721642103
                   HHHHHHHHHCTGGGTTTSEESSSSSEEEES-HHHHHHHTHHHHSTT--HHHHHHHHHHTTEEE---SSBTTTTXTTSEEEEESXXXXXXXXXXXXXX CS
  HSF_DNA-bind   2 FlkklyeiledeelkeliswsengnsfvvldeeefakkvLpkyFkhsnfaSFvRQLnmYgFkkvkdeekkskskekiweFkhksFkkgkkellekik 98 
                   Fl+k++ei++d+ ++ ++sws + +sf+++d+ ef++++LpkyFkh+nf+SF+RQLn YgFkkv++++         weF+++ F+ gkk+ll++ik
   AT5G54070.1  72 FLRKTFEIVDDKVTDPVVSWSPTRKSFIIWDSYEFSENLLPKYFKHKNFSSFIRQLNSYGFKKVDSDR---------WEFANEGFQGGKKHLLKNIK 159
                   9********************999*****************************************999.........******************** PP

                   XXXXX CS
  HSF_DNA-bind  99 rkkse 103
                   r++++
   AT5G54070.1 160 RRSKN 164
                   **985 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SMARTSM004152.1E-5168161IPR000232Heat shock factor (HSF)-type, DNA-binding
SuperFamilySSF467853.94E-3169161IPR011991Winged helix-turn-helix DNA-binding domain
Gene3DG3DSA:1.10.10.108.9E-3570161IPR011991Winged helix-turn-helix DNA-binding domain
PRINTSPR000561.4E-177295IPR000232Heat shock factor (HSF)-type, DNA-binding
PfamPF004475.6E-3072161IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000561.4E-17110122IPR000232Heat shock factor (HSF)-type, DNA-binding
PROSITE patternPS004340111135IPR000232Heat shock factor (HSF)-type, DNA-binding
PRINTSPR000561.4E-17123135IPR000232Heat shock factor (HSF)-type, DNA-binding
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043565Molecular Functionsequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0007632developmental stageseed maturation stage
Sequence ? help Back to Top
Protein Sequence    Length: 331 aa     Download sequence    Send to blast
MTAIPNVVDI ESSSSSLCQE TATETVTVER GSSDSSSKPD DVVLLIKEEE DDAVNLSLGF  60
WKLHEIGLIT PFLRKTFEIV DDKVTDPVVS WSPTRKSFII WDSYEFSENL LPKYFKHKNF  120
SSFIRQLNSY GFKKVDSDRW EFANEGFQGG KKHLLKNIKR RSKNTKCCNK EASTTTTETE  180
VESLKEEQSP MRLEMLKLKQ QQEESQHQMV TVQEKIHGVD TEQQHMLSFF AKLAKDQRFV  240
ERLVKKRKMK IQRELEAAEF VKKLKLLQDQ ETQKNLLDVE REFMAMAATE HNPEPDILVN  300
NQSGNTRCQL NSEDLLVDGG SMDVNGRIEI E
3D Structure ? help Back to Top
Structure
PDB ID Evalue Query Start Query End Hit Start Hit End Description
2ldu_A4e-216916117120Heat shock factor protein 1
5d5u_B5e-216916126129Heat shock factor protein 1
5d5v_B5e-216916126129Heat shock factor protein 1
5d5v_D5e-216916126129Heat shock factor protein 1
Search in ModeBase
Nucleic Localization Signal ? help Back to Top
NLS
No. Start End Sequence
1154160LKNIKRR
Expression -- Microarray ? help Back to Top
Source ID E-value
GEO3341883790.0
Genevisible248188_at0.0
Expression AtlasAT5G54070-
AtGenExpressAT5G54070-
ATTED-IIAT5G54070-
Expression -- Description ? help Back to Top
Source Description
UniprotDEVELOPMENTAL STAGE: Starts to be expressed in seeds from 18 days after pollination (DAP) to reach the highest expression in dry seeds. Expression is strongly reduced after only 2 hours of seed imbibition, declines drastically after 6 hours, but can be detected at a very low level even after 10 hours. {ECO:0000269|PubMed:17220197}.
Functional Description ? help Back to Top
Source Description
TAIRA member of Heat Stress Transcription Factor (Hsf) family. Not responding to heat stress. Is regulated by the seed-specific transcription factor ABI3. In turn, it regulates other heat stress proteins including Hsp17.4-CI, Hsp17.7-CII and Hsp101 during seed maturation.
UniProtSeed-specific transcriptional regulator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Seems to be specialized for the developmental expression of heat shock protein (HSP) genes during seed maturation. Activated by ABI3. {ECO:0000269|PubMed:17220197}.
Function -- GeneRIF ? help Back to Top
  1. Developmental expression of HsfA9 is regulated by the seed-specific transcription factor ABSCISIC ACID-INSENSITIVE3 (ABI3).
    [PMID: 17220197]
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT5G54070.1
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- ATRM (Manually Curated Upstream Regulators) ? help Back to Top
Source Upstream Regulator (A: Activate/R: Repress)
ATRM AT3G24650 (A)
Regulation -- ATRM (Manually Curated Target Genes) ? help Back to Top
Source Target Gene (A: Activate/R: Repress)
ATRM AT1G74310(A)
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT5G54070
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0652260.0AY065226.1 Arabidopsis thaliana unknown protein (At5g54070) mRNA, complete cds.
GenBankAY0965520.0AY096552.1 Arabidopsis thaliana unknown protein (At5g54070) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_200218.10.0heat stress transcription factor A-9
SwissprotQ9LVW20.0HSFA9_ARATH; Heat stress transcription factor A-9
TrEMBLD7MTN90.0D7MTN9_ARALL; Putative uncharacterized protein
STRINGAT5G54070.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM74981940
Representative plantOGRP1180756
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Nover L, et al.
    Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?
    Cell Stress Chaperones, 2001. 6(3): p. 177-89
    [PMID:11599559]
  3. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  4. Kotak S,Vierling E,Bäumlein H,von Koskull-Döring P
    A novel transcriptional cascade regulating expression of heat stress proteins during seed development of Arabidopsis.
    Plant Cell, 2007. 19(1): p. 182-95
    [PMID:17220197]
  5. Prieto-Dapena P,Casta
    The ectopic overexpression of a seed-specific transcription factor, HaHSFA9, confers tolerance to severe dehydration in vegetative organs.
    Plant J., 2008. 54(6): p. 1004-14
    [PMID:18315542]
  6. Guo J, et al.
    Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis.
    J Genet Genomics, 2008. 35(2): p. 105-18
    [PMID:18407058]
  7. Babu M,Griffiths JS,Huang TS,Wang A
    Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection.
    BMC Genomics, 2008. 9: p. 325
    [PMID:18613973]
  8. Nakashima K, et al.
    Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy.
    Plant Cell Physiol., 2009. 50(7): p. 1345-63
    [PMID:19541597]
  9. Jin J, et al.
    An Arabidopsis Transcriptional Regulatory Map Reveals Distinct Functional and Evolutionary Features of Novel Transcription Factors.
    Mol. Biol. Evol., 2015. 32(7): p. 1767-73
    [PMID:25750178]